Water relations, cuticular transpiration, and bud characteristics of air-slit containerized Picea glauca seedlings in response to controlled irrigation regimes

Abstract

White spruce seedlings (Picea glauca (Moench) Voss) were grown in air-slit containers (IPL 25-350A) in a tunnel under four different irrigation regimes (IR-15%, IR-30%, IR-45%, and IR-60%, v/v; (cm H2O)3·(cm substrate)–3). At the end of the first growing season the water-relation variables of the shoots were determined. Seedling morphology, the rates of cuticular transpiration and terminal bud development, as well as the number of needle primordia were also measured. Irrigation regime had no significant effect on any of the water-relation variables. Seedlings grown under the IR-15% were smaller and completed bud development more quickly than seedlings grown under IR-30%, IR-45%, and IR-60%. The formation of needle primordia was enhanced under IR-30%. Both the irrigation regime and the amount of time the detached shoots were left to transpire had a significant effect on the rate of cuticular transpiration. A comparison of the water-relation variables at the end of the first (1998) and second (1999) growing seasons showed that the younger seedlings had more negative osmotic potential at tissue saturation and greater maximum modulus of elasticity values. None of the other water-relation variables were significantly affected by seedling age, but the absolute values of all the variables were greater in the younger (1+0) seedlings.