Stomatal guard cell length as an indictor of ploidy in microspore-derived plants of barley

Abstract

A cytological examination of 96 microspore-derived barley genotypes indicated that haploid and diploid progeny were produced in approximately equal proportions. In order to discriminate between haploid and nonhaploid derivatives, indirect ploidy evaluation techniques were examined. Stomatal guard cell length provided a convenient and reliable method for classifying microspore-derived plants into haploid, diploid, and tetraploid groups.Key words: haploid, microspore, ploidy level, guard cell, stomata, Hordeum vulgare L.