ISOLATION OF 22-DEHYDROCHOLESTEROL FROM SCALLOP

Abstract

22-Dehydrocholesterol has been isolated from the sterols of the scallop Placopecten magellanicus (Gmelin). The azoyl esters of the unsaponifiable fats were separated into four zones by chromatography. The Δ22-sterol accounted for 31.3% of the chromatographic γ-zone and 14.1% of the total sterol fraction. The homogeneity and structure of the isolated sterol were established by gas chromatography. Further confirmation of its identity was afforded by comparison of its physical properties with synthetic 22-dehydrocholesterol and with 22-dehydrocholesterol isolated from a species of red algae.