OBLIGATELY PSYCHROPHILIC YEASTS FROM THE POLAR REGIONS

Abstract

Sixty-seven pure cultures of psychrophilic bacteria and yeasts were isolated from polar ice, snow, soil, and other materials. Low temperatures were maintained during all phases of the isolation procedure to avoid possible destruction of obligate psychrophiles. Four of the cultures, all yeasts, proved to be obligate psychrophiles. Three are species of Candida and one of Torulopsis. They grew in the range of 0 °C to 20 °C and the optimum growth temperature was 15 °C. They died rapidly when exposed to temperatures of 30 °C or 40 °C.The rate of fermentation of glucose by psychrophilic Candida sp. P16 was higher than that of mesophilic Saccharomyces cerevisiae at temperatures below 25 °C. At and above 25 °C, this rate relationship was reversed and at 35 °C all fermentative activity of the psychrophilic yeast was destroyed while that of the mesophile was maximal. Oxidative activity of Candida sp. P16 was much less sensitive to 35 °C, which suggests that fermentation and oxidation proceed by different pathways.The extent of occurrence of obligately psychrophilic microorganisms in nature is discussed.