Synthesis and chemiluminescence of new derivatives of isoluminol

Abstract

In an attempt to improve the sensitivity of luminescent immunoassays, we have prepared some new isoluminol, 7-(N,N-dialkylamino)-5-methyl-2,3-dihydrophthalazine-1,4-diones by means of a novel procedure involving the cycloaddition of dienamines to maleic acid derivatives. These compounds are characterized by the presence of a methyl group at C-5 and give quantum yields three to five times greater than those of the most efficient isoluminols in use at present.