Author:
Moore-Landecker Elizabeth
Abstract
Pyronema domesticum (Sow. ex Gray) Saccardo was cultured at temperatures ranging from 5 to 10 °C; relative humidities (RH) from 100% to near 0%; on an inorganic salts medium supplemented with 0.5–1.0 g dextrose, 0.5–3.5 g Casamino acids, 10 g charcoal, or 20 g inulin per litre; and on potato dextrose agar. The effects of these different cultural conditions on apothecial morphogenesis were evaluated. Fully expanded, globose ascogonia predominated in the control, at 20–25 °C, 93–100% RH, 0.5 g dextrose, or 0.5 g Casamino acids/litre. Undifferentiated (without a trichogyne) or poorly differentiated ascogonia predominated at extremely unfavorable conditions (5–10 and 30 °C; about 0–20% RH; 3.5 g/litre Casamino acids, 5–6.5 × normal concentration of inorganic salts). These latter conditions sometimes prevented fusion and (or) plasmogamy. Under all remaining conditions, the ascogonia were predominately clavate to obovate with a delimited trichogyne, and the normal sexuality was unaffected. Morphological abnormalities were common among the poorly differentiated gametangia but less common among those that were better differentiated. Ascogenous hyphae were produced by all types of ascogonia. There was generally some reduction in ascus production, and asci with mature ascospores were not formed at 5 or 30 °C, near 0% RH, on 3.5 g Casamino acids/litre, or on 3.5–6.5 × inorganic salts. There was some reduction in the sterile system under the most extreme conditions, and a palisade of paraphyses was not formed at 5–10 °C, and 4.5–6.5 × inorganic salts. Sclerotia were formed only on potato dextrose agar.
Publisher
Canadian Science Publishing
Cited by
14 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献