Abstract
When sap from tobacco, infected with potato X virus, was dialyzed against 0.05 M and then against 0.005 M sodium citrate, no aggregation of the virus was detected after ultracentrifugation. Flow birefringence measurements were made to compare virus preparations obtained by ultracentrifugation of untreated sap and of sap that had been treated with various buffers. Only versene and citrate were found to prevent aggregation. The influence of pH in the range 5.7–7.3 was very slight. Possible explanations of this effect are discussed.Nitrogen and phosphorus content and the sedimentation constant of the purified, non-aggregated virus are reported.
Publisher
Canadian Science Publishing
Subject
Organic Chemistry,General Chemistry,Catalysis
Cited by
39 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献