Author:
Bousquet Jean,Simon Luc,Lalonde Maurice
Abstract
A simple protocol for the extraction of total DNA from minute amounts of tissues and subsequent amplification of specific sequences by polymerase chain reaction is presented. The method is applicable to a wide variety of vegetative tissues such as leaves, single needles and rootlets, cell suspensions, and also single sexual embryos and megagametophytes derived from a variety of gymnosperms and perennial angiosperms. Amplification of DNA is shown using pairs of primers specific to genes that encode the small ribosomal subunit.
Publisher
Canadian Science Publishing
Subject
Ecology,Forestry,Global and Planetary Change
Cited by
180 articles.
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