Rapid release of peroxidase by peanut cells in suspension culture

Abstract

Peanut cells in a suspension culture were studied with regard to the process of peroxidase release and some ultrastructural features that appeared to relate to this process. By means of gel filtration on Sephadex G-200 it was established that protein released into the medium had the same molecular weight as the peroxidase found in this medium. From information gained through the electron-microscopic studies and from incorporation of labeled leucine, it may be deduced that the metabolic rate of these cells is very high. Within 2 h after incubation with radioactive leucine it was noted that newly synthesized protein was released by the cells into the medium. This protein had also the same characteristics of peroxidase and its synthesis and subsequent appearance in the medium could be reduced by incubation in the presence of cycloheximide. A possible explanation for the presence of these large quantities of peroxidase is discussed.