Préparation d'un substrat de culture pour le strophaire (Stropharia rugoso-annulata) par trempage de résidus ligno-cellulosiques agricoles

Abstract

Because of the enzymatic make-up of Stropharia, a cultivated and edible mushroom, it is capable of using agricultural wastes as substrates for growth. Different substrates and methods of preparation are compared in order to improve the resistance of the mushroom to fungal contamination by reducing soluble carbon and nitrogen content. The substrates (wheat straw, corn stalk, or wastes from pea plants) were soaked at various temperatures and durations in order to eliminate soluble nutrients. High temperatures are more efficient for a rapid depletion of free nitrogen compounds. The best substrate used for the first time in mushroom cultivation was the agricultural waste from Pisum. Because of its naturally high content of soluble nutrients, this substrate needs to be soaked in hot water to reduce contaminations. Yields of Stropharia (220 g/kg) are higher and more regular than those obtained on conventional substrate (straw). On corn stalk, a high contamination rate was found, as well as relatively poor yields.Key words: mushroom, Stropharia, substrate, waste, yield, fungal contamination.