Fine structure of megagametophyte development in Zea mays

Abstract

Polygonum-type embryo sac development was examined in Zea mays with light, fluorescence, and electron microscopy. Megasporocyte polarity is expressed in organelle distribution, aniline blue wall fluorescence, and chalazal location of plasmodesmata. In the meiotic cytoplasm concurrent with the sporophyte–gametophyte transition, ribosome concentrations are low, and membrane-bound organelles are simplified. The functional megaspore is the largest and most chalazal cell of the resultant triad or tetrad. Aniline blue fluorescence is intense in nonfunctional megaspore walls, whereas in the functional megaspore intensity decreases from the chalazal end to that in nucellar walls. Throughout functional megaspore and two-nucleate megagametophyte, ribosome concentrations, organelle numbers, and structural complexity increase. The transition from vegetative to reproductive gametophytic growth presumably follows this biosynthetic period. In the four-nucleate stage, a second phase of mitochondrial simplification coincides with the formation of an extensive lytic vacuole containing evidence of possible organelle and cytoplasmic membrane incorporation. Near the close of intensive lytic activity, compartmentalization of lytic areas contributes to creating a prominent vacuole in the central cell as synergids, egg, and antipodals differentiate.