ELECTROPHORESIS OF HISTONES ON POLYACRYLAMIDE GELS-Reference-Cited by-同舟云学术

ELECTROPHORESIS OF HISTONES ON POLYACRYLAMIDE GELS

Published:1963-01-01 Issue:1 Volume:41 Page:2507-2516
ISSN:0576-5544
Container-title:Canadian Journal of Biochemistry and Physiology
language:en
Short-container-title:Can. J. Biochem. Physiol.

Author:

Driedger A.¹,Johnson L. D.¹,Marko A. M.¹

Affiliation:

1. Division of Biology and Health Physics, Atomic Energy of Canada Limited, Chalk River, Ontario, and the Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan

Abstract

Histories extracted from various organs of the rat have been fractionated by electrophoresis on Polyacrylamide gels. The most convenient reagents to form the gel were selected and the effects of varying concentrations of these reagents on the resolution of histones were investigated. Optimum conditions for the separation of histones were found to be the same as those for starch gel electrophoresis. More consistent results were obtained with the use of Polyacrylamide gels because these gels were equilibrated to constant pH and ionic strength prior to electrophoresis and the gels were electrophoretically destained to demonstrate the histone bands.

Publisher

Canadian Science Publishing

Subject

General Medicine

Link

http://www.nrcresearchpress.com/doi/pdf/10.1139/y63-280

Reference11 articles.

1. ZONE ELECTROPHORESIS OF CALF THYMUS HISTONE IN STARCH GEL

2. Acrylamide Gel as an Electrophoresis Medium

3. ALCOHOL LEVELS IN BODY FLUIDS AFTER INGESTION OF DISTILLED SPIRITS

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