Author:
Vijayakumar P.,Ross W.,Reese E. T.
Abstract
A simple adsorption and elution of the trehalase of Trichoderma reesei on bentonite increased the specific activity 70–80 times, with a recovery of 90%. This α,α-trehalase has an optimum pH of 4.4, a pl of 5.7, a Km of 3.1 × 10−3 M, and a specific activity of 50 μmol/mg. min−1.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
13 articles.
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2. Catalytic Reaction Mechanism Based on α-Secondary Deuterium Isotope Effects in Hydrolysis of Trehalose by European Honeybee Trehalase;Bioscience, Biotechnology, and Biochemistry;2009-11-23
3. Molecular Cloning of cDNA for Trehalase from the European Honeybee,Apis melliferaL., and Its Heterologous Expression inPichia pastoris;Bioscience, Biotechnology, and Biochemistry;2007-09-23
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5. Purification and Identification of the Essential Ionizable Groups of Honeybee, Apis mellifera L., Trehalase;Bioscience, Biotechnology, and Biochemistry;2001-01