Author:
Abbo S.,Miller T. E.,Reader S. M.,Dunford R. P.,King I. P.
Abstract
Hybridization sites of an rDNA probe coding for the 18S, 5.8S, and 26S genes were detected on lentil and chickpea somatic chromosomes using fluorescent in situ hybridization. One pair of hybridization sites was detected in cultivated lentil Lens culinaris L. and wild lentil L. orientalis (Boiss.) Hand.-Mazz., and in both the hybridization sites of the ribosomal probe correspond to the secondary constriction. In cultivated chickpea Cicer arietinum three pairs of rDNA sites were detected and in the wild C. reticulatum two pairs were detected. The karyotypic relationship between the cultivated C. arietinum and its wild progenitor C. reticulatum is discussed.Key words: chickpea, lentil, rDNA sites, nucleolus organizer region, fluorescent in situ hybridization, primer-mediated in situ hybridization.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,General Medicine,Biotechnology
Cited by
37 articles.
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