Abstract
Plantlets were regenerated from cultures established from Pinus sylvestris (Scots pine) meristematic tissue. Seedling explants were first stimulated to develop axillary buds. Developing axillary buds produced numerous new meristems that gave rise to globular adventitious buds located along the needles on half-strength modified Murashige and Skoog medium supplemented with coconut milk and 6-benzylaminopurine. A histological study showed that these new buds originated from dedifferentiated mesophyll and epidermal tissues of the needles. Some of these buds were used for the regeneration of whole plantlets, others were excised and transferred to woody plant medium, on which calli developed at the bases of the microcuttings These calli were organogenic when subcultured on a hormone-free medium and initiated a large number of rooted plantlets that showed high potential to multiply themselves indefinitely. This is the first report of regeneration of Scots pine from a subculturable organogenic line. Mycorrhizae were initiated for both types of plantlets with Hebeloma cylindrosporum on a perlite substrate under fully controlled conditions. Mycorrhizae improved the transfer of the plantlets to ex vitro conditions. Key words: organogenesis, mycorrhizae, tissue culture, Pinus sylvestris.
Publisher
Canadian Science Publishing
Cited by
17 articles.
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