IN VIVO INCORPORATION OF P32INTO CHICKEN SERUM PHOSPHOLIPIDS

Abstract

Chicken serum phospholipids have been separated by means of silicic acid column chromatography into phosphatidyl ethanolamine, lecithin, sphingomyelin, and lysolecithin. Their concentration in serum is 12, 68, 10, and 9% respectively of the total of 93.0 mg (average) phospholipid P per 100 ml. The maximum specific activity of serum phospholipids is attained 8 hours after the administration of a dose of inorganic P32. The rate of P32incorporation into serum phospholipids, as studied by means of silicic acid column chromatography, is of the following order: phosphatidyl ethanolamine > lecithin > lysolecithin > sphingomelin.Lysophosphatidyl ethanolamine has been isolated from chicken serum by means of silicic acid impregnated paper chromatography. It represents about 4% of total chicken serum phospholipids. It gave a glycerol to phosphorus ratio of 1.0 and fatty acid ester to phosphorus ratio of 1.03. It also showed a strong hemolytic activity on sheep erythrocytes, and its specific activity 8 hours after the injection of inorganic P32reached about 25% of that for phosphatidyl ethanolamine.