Somatic embryogenesis in tissue cultures of Liriodendrontulipifera

Abstract

Tissue cultures of yellow poplar (Liriodendrontulipifera L.) were initiated from immature and mature zygotic embryos. Nodular embryogenic callus developed from a low percentage of the cultures initiated from immature embryos on solid media supplemented with 2,4-dichlorophenoxyacetic acid, 6-benzyladenine, and casein hydrolysate. Embryoids differentiated from these culture lines within 1 month following transfer of embryogenic callus to hormone-free solid media. Although most embryoids appeared abnormal, embryoids with well-formed cotyledons and radicles were capable of developing into normal plantlets.