Author:
Lynch Thomas J.,Henney Jr. Henry R.
Abstract
Carbohydrate metabolism in the growing plasmodial phase of Physarum flavicomum was studied in partially defined media using the radiorespirometric technique and specifically labeled 14C-substrates. The Embden–Meyerhof–Parnas (EMP) – tricarboxylic acid cycle (TCA) and the pentose phosphate pathways are the routes by which glucose is used by this myxomycete. The replacement of the usual citrate–phosphate buffer by succinate–phosphate results in a decreased uptake of 14C-glucose from the medium and a corresponding decline in the rate of interval 14CO2 evolution. The addition of an inorganic nitrogen source (ammonium nitrate) to the medium also decreases the rate of carbohydrate metabolism and alters the relative participation of the pathways by favoring the EMP–TCA. Supplementing the medium with cyclic-3′-5′-adenosine monophosphate produces a transient stimulation of the rate of metabolism by the EMP–TCA. The Plasmodium is relatively impermeable to gluconate and pyruvate and does not readily metabolize amino acids.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
10 articles.
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