Effets stimulateurs des oligogalacturonates sur l'excrétion de polysaccharides acides par la suspension cellulaire de Silène

Abstract

The composition and the acidity of the exopolysaccharides excreted into the medium of suspension-cultured white campion cells (Silene alba (Miller) E.H.L. Krause) in presence of oligogalacturonates changed during the first hours after transfer into a new medium. The early increase in acidic polysaccharide secretion in the presence of oligogalacturonates fell quickly during the course of the culture cycle. This effect increased with the degree of polymerization of oligomers..The rapid nature of this response and the influence of size suggests a physicochemical mechanism. Furthermore, cell growth was partially inhibited by the oligogalacturonates. Key words: oligogalacturonates, cell growth, exopolysaccharides, Silene alba, suspension-cultured cells.