Molecular phylogeny of the protochordates: chordate evolution

Abstract

The deuterostomes are a monophyletic group of multicellular animals that include the Chordata, a phylum that exhibits a unique body plan within the metazoans. Deuterostomes classically contained three phyla, Echinodermata, Hemichordata, and Chordata. Protochordata describes two invertebrate chordate subphyla, the Tunicata (Urochordata) and the Cephalochordata. Tunicate species are key to understanding chordate origins, as they have tadpole larvae with a chordate body plan. However, molecular phylogenies show only weak support for the Tunicata as the sister-group to the rest of the chordates, suggesting that they are highly divergent from the Cephalochordata and Vertebrata. We believe that members of the Tunicata exhibit a unique adult body plan and should be considered a separate phylum rather than a subphylum of Chordata. The molecular phylogeny of the deuterostomes is reviewed and discussed in the context of likely morphological evolutionary scenarios and the possibility is raised that the ancestor of the Tunicata was colonial. In this scenario, the colonial tadpole larva would more resemble an ancestral chordate than the solitary tadpole larva. In contrast, the true chordates (vertebrates and cephalochordates) would have evolved from filter-feeding benthic worms with cartilaginous gill slits, similar to extant enteropneust hemichordates.