A modification of Heidenhain's iron-haematoxylin technique, as used to stain smears of Drosophila larval polytene chromosomes and neuroblast cells

Abstract

Acetic-acid-fixed smears of Drosophila larval salivary gland chromosomes and of neuroblast cells from the larval ganglion undergoing mitosis are prepared by a modification of Heidenhain's iron-haematoxylin technique, in which absolute methyl alcohol is the solvent of all reagents used in the staining process.