Genetic structure and transcriptional analysis of the arginine deiminase (ADI) cluster inLactococcus lactisMG1363

Abstract

In a recent proteomic analysis, we showed the overproduction of the ArcA and ArcB proteins in Lactococcus lactis MG1363 at low pH. The corresponding genes belong to the arcABD1C1C2TD2 cluster that encodes components of the arginine deiminase pathway. In this study, we characterized this cluster at the genetic level. Northern blot experiments showed the expression of at least seven transcripts, all induced by acidity. Tran script analysis using 5′RACE PCR (rapid amplification of cDNA ends polymerase chain reaction) in the arcB-arcD1intergenic region. In silico analysis identified nine stem-loop structures, all located in intergenic regions. Collectively, these data suggest a role for RNA processing and (or) premature termination in the differential expression of genes within the arcABD1C1C2TD2 cluster.Key words: lactic acid bacteria, acid stress, arginine catabolism.