MINOR PROTEINS IN HUMAN ERYTHROCYTES

Abstract

A study of 200 normal human blood samples, by the agar electrophoresis method, revealed that human haemolyzates contain two minor proteins in addition to the normal adult haemoglobin. The two minor proteins appearing in very low concentrations are non-haemoglobin in character, give a negative reaction to benzidine test, but a positive reaction to amido-schwarz. These two proteins have an electrophoretic mobility similar to that of γ-globulin of human serum, but appear as sharp distinct bands on the agar plate. The concentration of these non-haemoglobin components bear no correlation to the blood groups of the samples or to the haemoglobin concentration itself, but the components have some electrophoretic characteristics similar to some of the abnormal haemoglobins.