Author:
Yun Soon-Il,Yahya Ahmad RM,Malten Marco,Cossar Doug,Anderson William A,Scharer Jeno M,Moo-Young Murray
Abstract
The influence of peptidases on human interleukin-3 (rhIL-3) production by a recombinant Streptomyces lividans strain was investigated. The bacterium produced several general peptidases and tripeptidyl peptidases compromising the authenticity of rhIL-3. The level of peptidases depended on growth morphology. Growing S. lividans as compact pellets successfully reduced peptidase activity. Maximum general peptidase activity in pellet culture was delayed after maximum rhIL-3 concentration was achieved. The activity of the tripeptidyl peptidase was product (rhIL-3) associated.Key words: fermentation, interleukin-3, Streptomyces, tripeptidyl peptidase, proteases.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
2 articles.
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