Multiple nutritional currencies shape pregnancy in a large herbivore

Abstract

Nutritional condition embodies environmental conditions experienced by animals with survival and reproductive consequences. Body fat is often associated with ungulate fecundity; however, other nutritional currencies may limit fecundity. Using data from 129 moose (Alces alces (Linnaeus, 1758)) monitored over 429 moose-years, we examined the limiting role of multiple nutritional currencies on pregnancy rates while concurrently assessing the influence of age and prior reproduction. Females tended to be pregnant in successive years, suggesting differences in individual or habitat quality. However, probability of pregnancy declined with survival of calves from prior litters, indicating a reproductive cost to rearing offspring. Pregnancy was positively associated with serum iron (Fe), body fat, body mass, and serum protein. The best model predicting pregnancy included serum Fe, body fat, and age class, with serum Fe being the strongest single predictor. Moose with Fe concentrations considered deficient in cattle (Bos taurus Linnaeus, 1758) had pregnancy rates of 33%–35%, whereas 89%–91% of individuals with sufficient Fe were pregnant. We subsequently evaluated hypotheses concerning factors potentially limiting Fe concentrations, including Fe-deficient diet, chronic infection, parasitism, and malnutrition. The best supported hypothesis was energy and protein malnutrition constrained Fe stores. We conclude that subclinical anemia due to nutritional constraints can limit or indicate limits in moose fecundity.