Creation of artifactual internal membranes during fixation of Methanobacterium thermoautotrophicum
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Published:1987-10-01
Issue:10
Volume:33
Page:844-849
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ISSN:0008-4166
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Container-title:Canadian Journal of Microbiology
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language:en
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Short-container-title:Can. J. Microbiol.
Author:
Aldrich Henry C.,Beimborn Dieter B.,Schönheit Peter
Abstract
Methanogenic bacteria chemically fixed for electron microscopy contain internal membranes in the form of mesosomes and nucleoid vesicles, and the function of these membranes in methanogenesis and ATP synthesis has been speculated upon. Cells of Methanobacterium thermoautotrophicum fixed in phosphate-buffered 2.5% glutaraldehyde, 0.1% osmium tetroxide, or 0.5% glutaraldehyde – 2.5% formaldehyde always contain internal membranes. Cells freeze-fractured, freeze-substituted, or fixed in cacodylate-buffered 1% osmium tetroxide, 2.5% glutaraldehyde, or simultaneous glutaraldehyde–osmium lack internal membranes. We conclude that internal membranes in this species are artifacts arising during suboptimal fixation conditions.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
33 articles.
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