Quantitative trait locus mapping of rust resistance and agronomic traits in spring wheat

Abstract

Marker-assisted selection requires the identification of molecular markers associated with major genes and quantitative trait loci (QTLs). In this study, we used 167 doubled haploid lines derived from two unregistered spring wheat ( Triticum aestivum L.) parental lines that belong to the Canada Western Special Purpose wheat market class to map QTLs associated with five traits using inclusive composite interval mapping (ICIM). Using ICIM, the least-squares means phenotype data across three to four environments, and a genetic map of 2676 single-nucleotide polymorphisms (SNPs) out of the wheat 90K SNP array, we identified 10 QTLs associated with days to maturity (4A and 5B), plant lodging (4B, 5A, 5D, and 7D), grain yield (2D), leaf rust (4A), and stem rust (1A and 2B). Each QTL individually explained 6.0%–22.3% of the phenotypic variance and together accounted for 8.6%–38.2% of the total variance per trait. Two of the QTLs associated with rusts ( QLr.dms-4A and QSr.dms-1A) had a minor effect (6.0%–9.0%), whereas the second QTL for stem rust ( QSr.dms-2B) had a major effect (22.3%). Although chromosome 2B harbors multiple disease resistance QTLs, the physical location of QSr.dms-2B has not been reported in previous studies. Results from this study provide additional valuable information to wheat researchers; in particular, the area on chromosome 2B should be considered for future analyses.