Determination of Protein Digestibility in Atlantic Cod (Gadus morhua) with Internal and External Indicators

Author:

Lied E.,Julshamn K.,Braekkan O. R.

Abstract

Chromium(III)-oxide added to feed was determined by flame atomic absorption spectrophotometry in the same Kjeldahl digest as protein (N × 6.25). The recovery was 98.0% compared with only 40.0% using nitric/perchloric acid digestion. Samples of apparent indigestible protein in Atlantic cod (Gadus morhua) could be collected from the middle and distal part of the intestine 24 h after feeding a diet based on fillets of saithe (Pollachius virens). The level of reference substance and the apparent digestibility of protein indicated that feces samples from the rectum representing the feed could not be obtained until 72 h after the first feeding. Water-insoluble protein from the intestine and rectum accounted for 35–47% and 27%, respectively, of the total dry matter protein. Postdefecation loss of water-soluble nitrogenous components would therefore cause overestimation of protein digestibility. Chromium(III)-oxide and titanium(IV)-oxide were equivalent as external indicators. With titanium(IV)-oxide as indicator substance, calcium, iron, and zinc present in the feed were examined as internal reference substances. The gastric ratios of protein over calcium or zinc were compared with the corresponding ratios in the distal part of the intestine. The apparent protein digestibility agreed with the corresponding estimates obtained by titanium(IV)-oxide. Estimates of protein digestibility from capelin (Mallotus villosus) predating cod caught at sea using the gastrointestinal levels of calcium and zinc as internal reference substances corresponded well with estimates from experiments with cod fed whole capelin with titanium(IV)-oxide as external reference substance. The estimates indicate that samples of feces should be taken from the distal part of the intestine excluding the rectum. Because of large differences between individuals, a sufficient number of fish should be included in digestibility studies.Key words: cod, protein digestibility, digestibility indicators, feces sampling

Publisher

Canadian Science Publishing

Subject

Aquatic Science,Ecology, Evolution, Behavior and Systematics

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3