ICMSF methods studies. V. The influence of selective enrichment media and incubation temperatures on the detection of salmonellae in raw frozen meats

Abstract

Sixty lots of frozen raw meat were analyzed for Salmonella using preenrichment in lactose broth followed by selective enrichment in four different selective broths, each incubated at 35C and 43C. Incubation at 43C resulted in detection of more Salmonella-containing samples. The highest recovery was observed with selenite cystine at 43C. However, the data indicated that two selective broths should be used for maximum recovery. Tetrathionate broth without brilliant green was inferior to the three other selective media used, both at 35C and 43C. Using selenite cystine, tetrathionate brilliant green, and selenite brilliant green sulfa broths, a number of medium/temperature pairs gave Salmonella recoveries approaching that obtained from all eight medium/temperature variables combined.