Microinjection of fungal cells: a powerful experimental technique

Abstract

Microinjection is an effective method for introducing membrane-impermeant molecules into cells. As yet however, mycologists have made only limited use of this technique. Recent improvements in both equipment and methodology may change this situation as it is now possible to routinely microinject small turgid cells. In this paper I will review microinjection techniques and evaluate these with regard to fungal cells. The potential of microinjection for furthering our knowledge of fungal biology will be discussed. Key words: microinjection, fungi, oomycetes, F-actin, calcium.