Author:
Pristupa Z. B.,McAuley C. M.,Scrimgeour K. G.
Abstract
The polypeptide chain of folylpolyglutamate synthetase from beef liver has been isolated and partially characterized. This polypeptide has an apparent molecular weight of 73 000. Its amino-terminal residue is blocked. Amino acid analysis agrees with the hydrophobic properties and the pi (6.0) of this cytosolic enzyme. Polyclonal antibodies to the denatured enzyme have been prepared.Key words: folylpolyglutamate synthetase, folates, polyglutamates, electrophoresis, antisera.
Publisher
Canadian Science Publishing
Subject
Cell Biology,Molecular Biology,Biochemistry
Cited by
2 articles.
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