Abstract
A technique is described for the direct isolation and growth of individual vitally stained microorganisms which have been observed in soil preparations. Soil is stained with acridine orange and mounted on agar so that individual cells can be picked from the soil with a micromanipulation tool while the organism is being viewed with an ultraviolet fluorescence microscope. These cells are then grown in nutritive media to provide cultures for identification of the organisms and for studies of their nutritional, physiological, and morphological characteristics.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
17 articles.
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2. Nonmolecular Methods in Geomicrobiology;Geomicrobiology, Fifth Edition;2008-12-22
3. Methods in Geomicrobiology;Geomicrobiology, Fourth Edition,;2002-04-12
4. Microbial communities, activity and biomass;Agriculture, Ecosystems & Environment;1991-02
5. 10 Methods for Studying the Microbial Ecology of Soil;Methods in Microbiology;1990