Acute upregulation of PGC-1α mRNA correlates with training-induced increases in SDH activity in human skeletal muscle

Author:

Bonafiglia Jacob T.1,Edgett Brittany A.1,Baechler Brittany L.2,Nelms Matthew W.1,Simpson Craig A.3,Quadrilatero Joe2,Gurd Brendon J.1

Affiliation:

1. School of Kinesiology and Health Studies, Queen’s University, Kingston, ON K7L 3N6 Canada.

2. Department of Kinesiology, University of Waterloo, Waterloo, ON N2L 3G1, Canada.

3. Department of Emergency Medicine, Queen’s University, Kingston, ON K7L 3N6, Canada.

Abstract

The purpose of the present study was to determine if acute responses in PGC-1α, VEGFA, SDHA, and GPD1–2 mRNA expression predict their associated chronic skeletal muscle molecular (SDH–GPD activity and substrate storage) and morphological (fibre-type composition and capillary density) adaptations following training. Skeletal muscle biopsies were collected from 14 recreationally active men (age: 22.0 ± 2.4 years) before (PRE) and 3 h after (3HR) the completion of an acute bout of sprint interval training (SIT) (eight 20-s intervals at ∼170% peak oxygen uptake work rate separated by 10 s of recovery). Participants then completed 6 weeks of SIT 4 times per week with additional biopsies after 2 (MID) and 6 (POST) weeks of training. Acute increases in PGC-1α mRNA strongly predicted increases in SDH activity (a marker of oxidative capacity) from PRE and MID to POST (PRE–POST: r = 0.81, r2 = 0.65, p < 0.01; MID–POST: r = 0.79, r2 = 0.62, p < 0.01) and glycogen content from MID to POST (r = 0.60, r2 = 0.36, p < 0.05). No other significant relationships were found between acute responses in PGC-1α, VEGFA, SDHA, and GPD1–2 mRNA expression and chronic adaptations to training. These results suggest that acute upregulation of PGC-1α mRNA relates to the magnitude of subsequent training-induced increases in oxidative capacity, but not other molecular and morphological chronic skeletal muscle adaptations. Additionally, acute mRNA responses in PGC-1α correlated with VEGFA, but not SDHA, suggesting a coordinated upregulation between PGC-1α and only some of its proposed targets in human skeletal muscle.

Publisher

Canadian Science Publishing

Subject

Physiology (medical),Nutrition and Dietetics,Physiology,General Medicine,Endocrinology, Diabetes and Metabolism

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