The production and assay of Myxovirus influenza A/PR8 in suspension cultures of a continuous cell line of human origin (G2)
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Published:1971-10-01
Issue:10
Volume:17
Page:1335-1339
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ISSN:0008-4166
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Container-title:Canadian Journal of Microbiology
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language:en
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Short-container-title:Can. J. Microbiol.
Author:
Johnson C. Margaret,Westwood John C. N.
Abstract
High yields of influenza A/PR8, comparable to those obtained in the 10-day chick embryo, were obtained using a low input multiplicity of infection in suspension cultures of a continuous line of mammalian cells (G2). By using the hemadsorption differential (HAD) count, a technique combining hemadsorption with viable cell counting, the progress of virus production can be assayed and correlated with the metabolic condition of the cells.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology