Characterization and banding of mitotic chromosomes from two established cell lines of the German cockroach Blattella germanica

Abstract

A detailed analysis of karyology, chromosome measurement, and chromosome constitution in different cell populations from two established cell lines of the German cockroach Blattella germanica is provided. All 12 pairs of chromosomes in somatic cells are clearly identifiable. All chromosomes except No. 5 display distinct C- bands. The G- and Q-banding patterns are not consistent, but many cross bands were observed. The N-banding pattern is reproducible and quite similar to the C-banding pattern. The cells in vitro as compared with cells in vivo are found to be extremely suitable for analysis of chromosomes of this species.