Inoculum density, temperature, and genotype effects on in vitro growth promotion and epiphytic and endophytic colonization of tomato (Lycopersicon esculentum L.) seedlings inoculated with a pseudomonad bacterium

Abstract

The effects of inoculum density (0, 4.6 × 107, 4.2 × 108, and 8.8 × 108 cfu∙mL−1), temperature (10, 20, and 30 °C), and plant genotype (cultivars Celebrity, Blazer, Scotia, and Mountain Delight) on bacterial colonization and plant growth promotion were investigated in a gnotobiotic system. An in vitro dual culture of tomato (Lycopersicon esculentum L.) plantlets and a Pseudomonas sp., strain PsJN, were used. Epiphytic (external) and endophytic (internal) bacterial populations were determined to evaluate plantlet colonization. Shoot and root biomass of bacterized plantlets was significantly higher (p ≤ 0.05) than that of nonbacterized controls. Growth promotion was best with inoculum densities of 3 × 108 – 7 × 108 cfu∙mL−1 at 20 °C, particularly in the early maturing cultivars Blazer and Scotia. Lower inoculum densities were required to maximize root growth (approximately 1 × 108 cfu∙mL−1) than shoot growth (approximately 3 × 108 cfu∙mL−1). Shoot surface populations did not vary with inoculum density or temperature, but the bacterium colonized the shoot exterior of cultivars Celebrity, Mountain Delight, and Scotia better than cultivar Blazer. The root surface populations increased linearly with increasing inoculum density (within a range of 107–108 cfu∙mL−1), decreased with increasing temperatures (from 10 to 30 °C), and were higher for the main season cultivar Celebrity than for cultivars Blazer, Scotia, and Mountain Delight. Populations of shoot endophytes did not vary with initial inoculum density or genotype but were affected by temperature; the highest colonization was at 10 °C. The number of root endophytes was also highest at 10 °C at the inoculum density of approximately 4 × 108 cfu∙mL−1 and did not vary with genotypes. The experiments clearly indicate that there was no relationship between root surface colonization and plant growth promotion. However, the range of inoculum levels (3 × 108 – 7 × 108 cfu∙mL−1) that promoted colonization of the inner root tissues (endophytic) also best promoted plant growth. A possible biostimulation threshold within the tissues of the inoculated plants under conditions favourable to the growth of tomato is proposed.Key words: Pseudomonas sp., tomato, colonization, growth promotion.