Morphological comparison of isolates of Phakopsora pachyrhizi from different areas of the world

Abstract

Isolates of Phakopsora pachyrhizi from Australia, Indian, the Philippines, Taiwan, and Puerto Rico were compared with respect to morphology and development of 'Wayne' soybean. The most extensive comparisons were made between the Puerto Rican and Taiwanese isolates; these were indistinguishable in their prepenetration, penetration, and early colonization phases. Examination of uredia of all isolates by light and scanning electron microscopy (SEM) revealed no differences in uredial morphology. All isolates were indistinguishable with respect to uredospore size, shape, and apparently the number and distribution of uredospore germ pores. The only difference observed was the appearance of germ pores; germ pores of the Puerto Rican isolate were easier to see by means of SEM than those of the four Eastern Hemisphere isolates, suggesting that the Puerto Rican isolate may have thinner germ pore plugs. This difference is not sufficient to consider the isolates as taxonomically distinct.