Silent sounds in the Andes: underwater vocalizations of three frog species with reduced tympanic middle ears (Anura: Telmatobiidae: Telmatobius)

Abstract

Underwater vocalization in anurans is restricted to a few, distantly related species. In some of them, sound is transmitted through tympanic and extra-tympanic pathways. Members of the Andean genus Telmatobius Wiegmann, 1834 lack a tympanic membrane, and earlier reports assumed the absence of vocalizations in the genus. We recorded underwater vocalizations and examined the middle-ear morphology in three species of Telmatobius with different lifestyles: Telmatobius oxycephalus Vellard, 1946 (semiaquatic, riverine); Telmatobius hintoni Parker, 1940 (markedly aquatic, riverine); Telmatobius culeus (Garman 1876) (fully aquatic, lacustrine). Males emit underwater calls, which in the three species are simple and stereotyped; they consist of a repeated train of notes, with a low fundamental frequency (309–941 Hz). In each of the three species, the tympanic membrane is absent and the tympanic cavity is extremely reduced or absent, whereas the opercular system is well developed. Our data, along with prior knowledge in other species of anurans, suggest that the species examined here probably perceived sound through extra-tympanic pathways. Given the limited knowledge about underwater calling in anurans, Telmatobius seems a logical candidate to study the functional and evolutionary bases of underwater hearing and tympanic middle-ear reduction in anurans.