Protein synthesis in different cell lines infected with orthoreovirus serotype 3: inhibition of host-cell protein synthesis correlates with accelerated viral multiplication and cell killing

Abstract

In this study, we examined the viral multiplication of human reovirus serotype 3 in mammalian cell lines of different species and tissue origins. All cell lines supported viral multiplication to a similar extent, although the kinetics was significantly different in some of these lines. The appearance of infectious virus was delayed in few cell lines. These cell lines also exhibited a much slower rate of cell killing, and in contrast to the others, did not exhibit an inhibition of the synthesis of host-cell proteins. We conclude that the inhibition correlates with rapid cell killing and viral multiplication, while being unrelated to efficient total viral production.Key words: reovirus, protein synthesis inhibition, viral multiplication, cytopathic effects.