Hemolymph homeostasis in relation to diel feeding activity and microclimate in the prototypal land isopodLigia pallasii

Abstract

Twenty-four-hour variability in hemolymph osmolality was assessed in a field population of the semiterrestrial isopod Ligia pallasii and related to feeding activity and microenvironmental conditions of relative humidity (RH) and temperature in the species' habitats. Hemolymphs were also analyzed for levels of ammonia and glucose as indicators of digestive and absorptive activities. Comparative data on hemolymph osmolality and microhabitat conditions were also obtained for several ligiid species in Hawai'i. Late-morning peaks in hemolymph osmolality in L. pallasii were coincidental with peaks in ammonia and glucose and with predicted times of digestion/absorption based on observed feeding activity in the population, suggesting that the elevations in osmolality were due to salt-loading from the species' seaweed diet. Uniformity of microenvironmental conditions of RH and temperature in Ligia's supralittoral habitat, whether temperate or tropical, and regardless of time of day or localized weather conditions, suggests that dietary salt-loading is likely the greatest challenge to osmotic homeostasis in ligiids. This feature and other aspects of hemolymph osmolality in ligiids are discussed in the context of colonization of land by prototypal terrestrial isopods.