Characterization in vitro of H+ secretion and H+:Na+ exchange by an organism normally inhabiting a CO2-rich environment: Hymenolepis diminuta (Cestoda) in the rat intestine

Abstract

H+ and Na+ transport by the intestinal parasite Hymenolepis diminuta were studied in vitro. The flatworms acidified the ambient fluid by secreting H+ and the acidification could not be correlated with organic acid excretion. Ambient CO2-independent H+ secretion was attributed to protons of metabolic origin: dephosphorylation reactions and ionization of organic acids within the tissues. Ambient CO2-dependent H+ secretion was attributed to protons produced as a result of the hydration of CO2 within the tissue and to the stimulation of anaerobic metabolic pathways by CO2 acting as a cosubstrate in energy metabolism. Studies in which Na+ uptake was stimulated by CO2 or glucose and inhibited by ouabain, amiloride, or Na+ replacement suggested a partial direct coupling of Na+ absorption and H+ secretion but the different activation energies and the effect of buffer anions other than HCO3− suggested an indirect interaction. Various interactions were considered, including the effect of CO2 and intracellular ion concentrations on metabolic reactions leading to the supply of protons for H+ secretion and energy for ion transport.