The Tris-Catalyzed Isomerization of Potassium D-Glucose 6-O-sulfate

Abstract

Potassium D-glucose 6-O-[35S]sulfate was partially converted by Tris (≥25 mM, pH 7.5) to 35SO42−, fructose, and a 35S-labelled compound which was tentatively identified as fructose 6-O-sulfate. The isomerization reaction was also catalyzed by ethanolamine and 2-dimethylaminoethanol but not by glycine, triethanolamine, or pentaerythritol. The significance of these findings in relation to kinetic studies of the enzyme glycosulfatase is discussed.