Evaluation of the combined temperature and relative humidity preferences of the Colombian terrestrial salamander Bolitoglossa ramosi (Amphibia: Plethodontidae)

Abstract

Temperature and humidity are critical factors for terrestrial lungless salamanders, as their body temperatures are largely determined by the environmental temperature and require moisture to sustain cutaneous respiration. Herein, we evaluated the preference of Bolitoglossa ramosi Brame and Wake, 1972 between a high temperature and a high relative humidity (RH), the influence of temperature on RH preferences, and the influence of RH on the thermal preferences. This study was performed in a field location in the municipality of Líbano, Tolima, Colombia. There, on different nights, we collected 84 adult B. ramosi and carried out the preference experiments, using aluminum troughs with different thermal and RH gradients. We found that between high temperature and high RH, B. ramosi preferred high RH. However, B. ramosi selected high temperatures when the gradient had a high RH and low temperatures when the gradient had a low RH. These results show that B. ramosi is able to thermoregulate and hydroregulate. Nevertheless, hydroregulation seems to be more important than thermoregulation because B. ramosi always selected the high RH gradients, while their thermal selection relied on the hydric environment.