Inheritance and linkage relationships of seed peroxidase loci in hexaploid oat (Avena sativa L.)

Abstract

Polyacrylamide gel electrophoresis has been used to investigate the inheritance and linkage relationships between anodal (PXA) and cathodal (PXC) seed peroxidases in hexaploid oat (Avena sativa L.). A total of 12 seed peroxidase loci (5 loci of PXA and 7 loci of PXC) were identified in three crosses. Only two Pxc loci (Pxc5 and Pxc7) were not linked to any peroxidase loci; the others were scored in three linkage groups. The order of the three loci assigned to one of the linkage groups was Pxc1–Pxa5–Pxc2. The order of loci in the other two linkages were Pxc4–Pxa1–Pxa3 and Pxc3–Pxa4–Pxa2. Also, the Pxc6 locus was shown to be linked to the Pxc3 locus. Considering that A. sativa is an allohexaploid, it can be proposed that the three peroxidase linkages represent homoeologous chromosomes.Key words: seed peroxidase, isozyme inheritance, linkage, Avena sativa.