Affiliation:
1. Laboratoire de Biotechnologies végétales Appliquées à l’Amélioration des cultures, Faculté des Sciences de Gabès, Cité Erriadh Zrig 6072 Gabès, Tunisia.
2. Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM) UMR 113 IRD/CIRAD/AGRO-M/UM-II, USC INRA Campus International de Baillarguet TA-A82/ J 34398 Montpellier CEDEX 5, France.
Abstract
Forty-two bacterial isolates from root nodules of Argyrolobium uniflorum growing in the arid areas of Tunisia were characterized by phenotypic features, RFLP, and sequencing of PCR-amplified 16S rRNA genes. The isolates were found to be phenotypically diverse. The majority of the isolates tolerated 3% NaCl and grew at temperatures up to 40 °C. Phylogenetically, the new isolates were grouped in the genera Sinorhizobium (27), Rhizobium (13), and Agrobacterium (2). Except for the 2 Agrobacterium isolates, all strains induced nodulation on Argyrolobium uniflorum, but the number of nodules and nitrogen fixation efficiency varied among them. Sinorhizobium sp. strains STM 4034, STM 4036, and STM 4039, forming the most effective symbiosis, are potential candidates for inoculants in revegetalisation programs.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
19 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献