Structure and development of larval antennae in embryos ofLytta viridanaLeConte (Coleoptera: Meloidae)

Abstract

At time of hatch (252–264 h at 25 ± 0.5 °C), each antenna in Lytta viridana has three flagellomeres, three extrinsic muscles, and 25 sensilla of five different types, including a large composite sensillum of 19 sensory units on flagellomere II. Each antenna evaginates from epidermis on either side the stomodaeum beginning at 16% of embryogenesis. At 21%, a cell near its apex divides into two pioneer neurons that move into its lumen and project their axons to the brain by 29%. Sensillar stem cells begin to emerge at 23%, those of the appendix within a large embryonic placode and, from 26 to 48%, divide asymmetrically to generate the neurons and accessory cells of each sensillum. Sensillar axonogenesis begins at 34%, the first axons contact the brain at 35%, and antennal glomeruli begin to form within the deutocerebra at 57%. At 35%, the trichogen cell of each sensillum begins to grow out and larval cuticle is deposited about these, beginning at 57%. Upon withdrawal of trichogen cytoplasm from within the appendix at 81%, the dendrites of each sensory unit grow into it and begin to branch. Functional aspects are addressed and the observations compared with the limited information available on embryos of other insects.