Acid and alkaline phosphatase activity in relation to egg-laying and aging in Callosobruchus analis (Fab.) (Coleoptera: Bruchidae)

Abstract

Acid and alkaline phosphatase (EC 3.1.3.2 and EC 3.1.3.1) activities have been studied during aging of male and female Callosobruchus analis (Fab.). Acid phosphatase activity was found to be much higher than alkaline phosphatase activity in both sexes throughout adult life. Both the enzymes demonstrate two peaks of activity, one on the 3rd day and the other on the 10th day of the adult life. The first peak coincides with egg-laying activity in the female, where the egg production is found to be at a maximum until the 3rd day. The peak for both enzymes on the 10th day may be responsible for mobilizing of metabolites before the death of insects.