A conformational study of the adducts of 2′-deoxythymidine and 2,2,6,6-tetramethyl-1,4-piperidone-N-oxyl by 1H and 13C nuclear magnetic resonance

Abstract

γ-Irradiation of oxygen-free, aqueous solutions of 2′-deoxythymidine in the presence of the organic nitroxide free radical, 2,2,6,6-tetramethyl-1,4-piperidone-N-oxyl (TAN), leads to a complex mixture of products in which the TAN moiety is linked to the C5 or C6 position of a 5,6-saturated thymine ring. Extensive 1H and 13C nmr data are provided for the eight TAN–dT adducts which are produced in the largest amounts. The results show that the conformational properties of the sugar moiety are dependent on the point of attachment of the TAN group and the configuration of the saturated thymine ring.