In vitro secondary spreading from dissociating blastoderm-derived embryonic tissues of the zebrafish, Brachydanio rerio

Abstract

Brachydanio rerio (Teleostei: Cyprinidae) blastoderm-derived embryos, deprived of their epidermis in in vitro culture, dissociate and their tissues disperse radially upon the culture substrate. The phenomenon, referred to as secondary spreading, follows primary epidermal membrane formation and dissociation and spans a period from 18 days to 40 days of culture at 24 °C.Secondary spreads and associated cell types and cellular phenomena are described, and medium conditioning, substrate modification, degeneration, and necrosis are discussed.