Development of monoclonal-antibody-based immunobinding assays to detect growth of Gliocladiumroseum, a potential bioprotectant on unseasoned lumber

Abstract

Gliocladiumroseum Bain 321U is a potential bioprotectant against sapwood stains for unseasoned western hemlock (Tsugaheterophylla (Raf.) Sarg.) and amabilis fir (Abiesamabilis (Dougl. ex Loud.) Dougl. ex J. Forbes) lumber. An immunostaining method was developed to monitor spore germination and growth of G. roseum 321U on lumber during microcosm and field investigations. Spores, mycelium, or fungal extracts were immobilized on polyethersulfone or polyvinylidene difluoride membranes. The membranes were then probed with the G. roseum monoclonal antibody (6A5), and the fungus was detected either by using a chromogenic substrate or by enhanced chemiluminescence.